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Attachment microbes antagonistic against Botrytis cinerea —biological control and scanning electron microscope studies in vivo
Author(s) -
COOK DARRYL W M,
LONG PETER G,
GANESH SIVA,
CHEAH LH
Publication year - 1997
Publication title -
annals of applied biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.677
H-Index - 80
eISSN - 1744-7348
pISSN - 0003-4746
DOI - 10.1111/j.1744-7348.1997.tb05177.x
Subject(s) - botrytis cinerea , biology , biological pest control , conidium , mycelium , hypha , pathogen , microbiology and biotechnology , colonisation , bacteria , botrytis , antagonism , botany , colonization , biochemistry , genetics , receptor
Summary Bacteria and yeasts, selected by an attachment assay for their ability to adhere to Botrytis cinerea hyphae or conidia, were evaluated for biocontrol potential against B. cinerea on excised tomato stems. Eight of the 12 bacteria and seven of the eight yeast isolates conferred 90% to 100% biocontrol activity when antagonist populations were applied at three to 80 times the pathogen inoculum density. Biocontrol was maintained at similar levels when biocontrol agent (BCA) application was delayed up to 48 h after pathogen challenge. Scanning electron microscopy showed extensive colonisation of B. cinerea mycelium or conidia by many of these isolates and also evidence of pathogen degradation. The biocontrol efficacy and potential for the assay are discussed with respect to cell‐to‐cell adhesion as a vehicle to deliver antagonistic mechanisms to highly specific pathogen sites.