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Comparison of the coat protein of groundnut rosette assistor virus with those of other luteoviruses
Author(s) -
SCOTT K P.,
FARMER MJ,
ROBINSON D J.,
TORRANCE L.,
MURANT A F.
Publication year - 1996
Publication title -
annals of applied biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.677
H-Index - 80
eISSN - 1744-7348
pISSN - 0003-4746
DOI - 10.1111/j.1744-7348.1996.tb07091.x
Subject(s) - biology , luteovirus , immunogen , coat protein , rosette (schizont appearance) , plant virus , open reading frame , nicotiana benthamiana , virology , capsid , gene , virus , genetics , monoclonal antibody , peptide sequence , antibody , rna , immunology
Summary The coat protein gene of groundnut rosette assistor virus (GRAV) was cloned and sequenced. The deduced amino acid sequences of the coat protein and of another protein encoded in a different, overlapping, reading frame resemble those of other luteoviruses. Four monoclonal antibodies against GRAV, prepared using denatured coat protein as immunogen, also reacted with some other luteoviruses in ELISA. Nevertheless, they will be useful as reagents for the identification of GRAV infections in groundnut.