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Characterisation of a new virus from escarole
Author(s) -
CRESCENZI A.,
NUZZACI M.,
STRADIS A DE,
COMES S.,
PIAZZOLLA P.
Publication year - 1996
Publication title -
annals of applied biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.677
H-Index - 80
eISSN - 1744-7348
pISSN - 0003-4746
DOI - 10.1111/j.1744-7348.1996.tb07090.x
Subject(s) - biology , acyrthosiphon pisum , myzus persicae , aphis gossypii , trialeurodes , virus , coat protein , botany , ultrastructure , pisum , mosaic virus , plant virus , virology , homoptera , rna , aphididae , aphid , pest analysis , biochemistry , gene
Summary A new virus associated with mosaic, yellowing and necrotic symptoms in escarole has been isolated recently in southern Italy. The virus, for which the name escarole mosaic virus (EMV) is proposed, was transmissible by mechanical methods, by seeds and probably by pollen but not by Acyrthosiphon pisum, Aphis gossypii, Myzus persicae, Trialeurodes vaporariorum or Frankliniella occidentalis. The virions showed a single coat protein of about 32 kDa and eight encapsidated RNA species. Viral preparations sedimented as four components in sucrose density gradients. Electron microscopy indicated the presence of spherical particles with a diameter of 25 nm. Ultrastructural investigations on infected tissues revealed the formation of atypical inclusion bodies.