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Identification of Heterodera cysts by SOD isozyme electrophoresis profiles
Author(s) -
MOLINARI S.,
EVANS K.,
ROWE J.,
RUSSELL M.
Publication year - 1996
Publication title -
annals of applied biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.677
H-Index - 80
eISSN - 1744-7348
pISSN - 0003-4746
DOI - 10.1111/j.1744-7348.1996.tb05759.x
Subject(s) - heterodera avenae , biology , isozyme , superoxide dismutase , heterodera schachtii , heterodera , polyacrylamide gel electrophoresis , hydrogen peroxide , gel electrophoresis , enzyme , biochemistry , microbiology and biotechnology , nematode , ecology
Summary. Superoxide dismutase (SOD), the enzyme which converts superoxide anions into hydrogen peroxide, has been found to be highly expressed in the cyst stage of a number of Heterodera species likely to occur together in the field. By examining isoforms of this enzyme in polyacrylamide gel electrophoresis, we have been able to show different SOD profiles in six common species of Heterodera (H. avenae, H. cruciferae, H. glycines, H. goettingiana, H. schachtii and H. trifolii ). The method is quick and therefore lends itself to routine use in a diagnostic laboratory.