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Rapid detection of cowpea aphid‐borne mosaic virus in cowpea seeds
Author(s) -
KONATE G.,
NEYA B J
Publication year - 1996
Publication title -
annals of applied biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.677
H-Index - 80
eISSN - 1744-7348
pISSN - 0003-4746
DOI - 10.1111/j.1744-7348.1996.tb05750.x
Subject(s) - vigna , biology , mosaic virus , cowpea mosaic virus , aphid , cultivar , horticulture , agronomy , virus , plant virus , botany , virology
Summary. Four cultivars of cowpea ( Vigna unguiculata [L]. Walp.) were infected with cowpea aphid‐borne mosaic virus (CABMV) by natural infection in field plots. Seeds taken from these plants were tested for the presence of the virus by ELISA and symptom observation on the plantlets grown from the seeds. A biotin/ streptavidin ELISA technique was used and found to be more sensitive than a standard ELISA protocol for detecting CABMV infection in seed. There was a good correlation between the ELISA detection of CABMV in tissue taken from single cowpea seeds and subsequent development of infected plants grown from the same seeds. The ELISA technique is reliable for selecting CABMV‐free stocks of cowpea seeds.