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Isolation and characterisation of a new Venezuelan strain of cassava common mosaic virus
Author(s) -
MARYS EDGLORIS,
IZAGUIRREMAYORAL MARÍA LUISA
Publication year - 1995
Publication title -
annals of applied biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.677
H-Index - 80
eISSN - 1744-7348
pISSN - 0003-4746
DOI - 10.1111/j.1744-7348.1995.tb06655.x
Subject(s) - biology , potexvirus , virology , virus , strain (injury) , antiserum , plant virus , alfalfa mosaic virus , endoplasmic reticulum , coat protein , rna , antibody , biochemistry , gene , anatomy , immunology
Summary A new strain of cassava common mosaic virus, designated C s CMV‐ve, was isolated from cassava (Manihot esculenta Crantz) plants growing in Venezuela. The 520 nm long semi‐flexuous virus particles sedimented in sucrose gradients as one component and contained a single coat protein molecule of c. 28 kDa, and a ssRNA of c. 2.1 times 10 6 . A single dsRNA species of c. 4.2 times 10 6 kDA was isolated from virus‐infected cassava leaves. In double antibody sandwich enzyme‐linked immunosorbent assay, C s CMV‐ve reacted strongly with antisera to C s CMV and potato virus X, but not with those to other typical members of the potexvirus group. Ultrastructural studies of C s CMV‐ve infected cells showed the presence of large bundles of inclusion bodies in the cytoplasm, a proliferation of the rough endoplasmic reticulum, and large crystals in the peroxisomes. C s CMV‐ve in crude buffered sap or in purified preparations was not mechanically transmitted to known hosts of the Chaya and Brazilian strains of C s CMV. Isolated virus RNA also failed to infect a range of test‐plant species. This is the first report of the presence of C s CMV in Venezuela.