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The detection of salivary enzymes of phytophagous Hemiptera: a compilation of methods
Author(s) -
MADHUSUDHAN V V,
TAYLOR G S,
MILES P W
Publication year - 1994
Publication title -
annals of applied biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.677
H-Index - 80
eISSN - 1744-7348
pISSN - 0003-4746
DOI - 10.1111/j.1744-7348.1994.tb04146.x
Subject(s) - biology , polyphenol oxidase , pectinase , invertase , biochemistry , catalase , saliva , enzyme , cellulase , peroxidase , amylase , hemiptera , ascorbic acid , superoxide dismutase , alkaline phosphatase , acid phosphatase , catechol oxidase , botany , food science
Summary The role of Hemipteran saliva and salivary enzymes is central to an understanding of the etiology of damage that these insects cause to plants. The dilute nature of the salivary secretions and the minute quantities in which they are often obtainable have made analysis and detection of salivary components very difficult. Such investigations in this laboratory have led us to formalise the techniques involved and we believe that the compilation of these methods presented herein may be useful to other research workers in this area. Methods are described for acid and alkaline phosphatase, esterase, /S‐glucosidase, carbohydrases, invertase, amylase, proteinase, pectinase, cellulase, catalase, peroxidase, catechol oxidase, superoxide dismutase and ascorbic oxidase.