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Multiplication and distribution of iris severe mosaic virus in secondarily‐infected iris bulbs after stress induced by heat and wounding
Author(s) -
VLUGT CORNELIA I M VAN DER,
LOHUIS HENDRICUS,
DIJKSTRA JEANNE,
MARQUART ARNOUD,
GOLDBACH ROB W.,
BOONEKAMP PIET M.
Publication year - 1993
Publication title -
annals of applied biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.677
H-Index - 80
eISSN - 1744-7348
pISSN - 0003-4746
DOI - 10.1111/j.1744-7348.1993.tb04930.x
Subject(s) - biology , bulb , virus , iris (biosensor) , iridaceae , basal plate (neural tube) , basal (medicine) , virology , latent virus , botany , genetics , pregnancy , fetus , computer security , computer science , placenta , insulin , biometrics , endocrinology
SUMMARY In freshly‐lifted iris bulbs infected with iris severe mosaic virus (ISMV), virus was not always detected in the basal plate and rarely in bulb scale tissue. After exposing the bulbs to stress (wounding or high‐temperature treatment) the sensitivity of virus detection was enhanced. The improved detection of viral antigen after local stress (wounding) coincided with an increase of viral RNA synthesis. When general stress (high‐temperature treatment) was applied, the virus could be reliably detected in the basal plate, and usually in vascular bundles and surrounding tissue. Virus was detected in the upper part of the bulb scale when such tissues were detached from the basal plate. Thus, virus must have been present in the scales in localised spots, albeit at a very low concentration, and multiplication is likely to be the main factor involved in the improved sensitivity of viral detection. The distribution of ISMV in the bulb after local or general stress treatment is discussed.