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Priming responses of leek ( Allium porrum L.) seeds to different dissolved oxygen levels in the osmoticum
Author(s) -
BUJALSKI W.,
NIENOW A. W.,
MAUDE R. B.,
GRAY D.
Publication year - 1993
Publication title -
annals of applied biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.677
H-Index - 80
eISSN - 1744-7348
pISSN - 0003-4746
DOI - 10.1111/j.1744-7348.1993.tb04058.x
Subject(s) - imbibition , germination , horticulture , biology , oxygen , priming (agriculture) , nitrogen , peg ratio , botany , chemistry , organic chemistry , finance , economics
Summary Leek seeds ( Allium porrum L.) cv. Winterreuzen were treated for 5 days in stirred bioreactors containing a ‐1.0 MPa polyethylene glycol (PEG 6000) solution. The level of dissolved oxygen was controlled from zero to approximately two and a half times that of saturation with respect to atmospheric air by bubbling through 0 2 /N 2 gas blends from 0% O 2 to 50% O 2 . The treatment with 0% O 2 (i.e. bubbling with N 2 ) gave no reduction in germination time compared to untreated seeds after allowing for the time for water imbibition. As the proportion of oxygen was increased, a rapid reduction was observed until a concentration equivalent to air was used. Further increases to the maximum, produced only a marginal further improvement. The reduction in germination times for seeds that had been cabinet dried was longer for all treatments due to the time required for re‐imbibition of water but the general response to priming was the same as with undried seeds. Only dried seeds treated with air or higher oxygen concentrations showed enhanced synchronicity of germination times. The percentage germination of seeds from all treatments was the same, including the treatment with nitrogen gas. These tests help to explain the recently reported advantages of using enriched air for seed priming and are one of the necessary protocols for ensuring satisfactory bulk priming.