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Improved detection of potato leafroll virus in dormant potato tubers and microtubers by the polymerase chain reaction and ELISA
Author(s) -
SPIEGEL SARA,
MARTIN R. R.
Publication year - 1993
Publication title -
annals of applied biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.677
H-Index - 80
eISSN - 1744-7348
pISSN - 0003-4746
DOI - 10.1111/j.1744-7348.1993.tb04052.x
Subject(s) - potato leafroll virus , biology , polymerase chain reaction , reverse transcription polymerase chain reaction , virus , potato virus y , reverse transcriptase , in vitro , virology , plant virus , gene , messenger rna , biochemistry
Summary The polymerase chain reaction (PCR) method was reliably used for detection of potato leafroll virus (PLRV) RNA in dormant tubers from field‐grown plants and in vitro‐propagated microtubers. A simple RNA extraction from tuber and microtuber tissue allowed reverse transcription and PCR amplification of viral sequences. It was also demonstrated that ELISA could be used reliably for detection of PLRV in crude sap extracts of dormant microtubers produced in vitro but not in tubers from field‐grown plants.