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Identification and classification of a resistance breaking strain of barley yellow mosaic virus
Author(s) -
BENDIEK J.,
DAVIDSON A. D.,
SCHULZE S. C.,
SCHELL J.,
STEINBISS H.H.
Publication year - 1993
Publication title -
annals of applied biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.677
H-Index - 80
eISSN - 1744-7348
pISSN - 0003-4746
DOI - 10.1111/j.1744-7348.1993.tb04051.x
Subject(s) - biology , virology , strain (injury) , genetics , anatomy
Summary A “resistance breaking” isolate of barley yellow mosaic virus‐2 (BaYMV‐2) was cloned as a cDNA and characterised. Restriction mapping and comparison with a German and a Japanese isolate of BaYMV (BaYMV‐G and BaYMV‐J) revealed a high level of restriction site conservation for RNAl and the majority of RNA2. However, in a box of approximately 600 nucleotides located on RNA2, striking differences in the restriction pattern could be identified. The nucleotide sequence of this box, as well as of the 3‘‐terminal region of RNA1 including the coat protein coding region, and the deduced amino acid sequences were analysed. Identity at the amino acid level was between 99.3% and 92.3% in comparison with the corresponding sequences of BaYMV‐G and BaYMV‐J, suggesting that BaYMV‐2 is closely related to BaYMV. Consequently, the classification of BaYMV‐2 as a “resistance breaking” strain of BaYMV is justified.