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Peroxidase from cultured carrot cells: affinity purification and characteristics
Author(s) -
ANANTHANARAYANAN T. V.,
SWAMY R. DORE
Publication year - 1991
Publication title -
annals of applied biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.677
H-Index - 80
eISSN - 1744-7348
pISSN - 0003-4746
DOI - 10.1111/j.1744-7348.1991.tb06095.x
Subject(s) - concanavalin a , peroxidase , biology , affinity chromatography , extracellular , callus , lectin , intracellular , biochemistry , enzyme , sepharose , suspension culture , cell culture , microbiology and biotechnology , botany , in vitro , genetics
Summary Peroxidase, from cultured carrot cells, has been isolated and purified over Concanavalin‐A‐Sepharose by lectin affinity chromatography. The enzyme protein registers a gradual enhancement in activity concomitant with the growth of callus. Extracellular (ECP) & intracellular (ICP) peroxidases have been demonstrated in suspension cultures of carrot.