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Characterisation of datura yellow vein virus, a newly described rhabdovirus from Australia
Author(s) -
THOMAS J. E.,
DIETZGEN R. G.
Publication year - 1991
Publication title -
annals of applied biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.677
H-Index - 80
eISSN - 1744-7348
pISSN - 0003-4746
DOI - 10.1111/j.1744-7348.1991.tb05634.x
Subject(s) - datura stramonium , biology , lycopersicon , solanaceae , solanum , datura , nicotiana tabacum , antiserum , virus , virology , solanum nigrum , polyclonal antibodies , potato virus x , botany , titer , myzus persicae , plant virus , aphid , antibody , biochemistry , immunology , gene
SUMMARY A previously undescribed sub‐group 2 rhabdovirus was isolated in Queensland from Datura stramonium with symptoms of vein yellowing, leaf distortion and reduced leaf size. Particles accumulated in the perinuclear space of infected cells of D. stramonium and measured 77 × 166 nm in preparations from sap. The virus was named datura yellow vein virus (DYVV) and was graft‐transmitted to several hosts in the Solanaceae including Lycopersicon esculentum, Nicotiana tabacum and Solanum melongena , but not to Capsicum annuum or Solanum tuberosum. DYVV was not transmitted by mechanical inoculation and no insect vector was found. Purified particles of DYVV contained four structural proteins with molecular weights of about 78, 47, 41 and 36 kd. The 78 kd protein bound the lectin concanavalin A, thus identifying it as the viral glycoprotein. DYVV was serologically distinct from 11 other rhabdoviruses belonging to both subgroups, including potato chlorotic stunt, potato yellow dwarf (2 isolates) and tomato vein yellowing viruses. The glycoprotein only of DYVV cross‐reacted with a polyclonal antiserum to sonchus yellow net virus.