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Purification and some properties of strawberry mottle virus
Author(s) -
YOSHIKAWA N.,
CONVERSE R. H.
Publication year - 1991
Publication title -
annals of applied biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.677
H-Index - 80
eISSN - 1744-7348
pISSN - 0003-4746
DOI - 10.1111/j.1744-7348.1991.tb05346.x
Subject(s) - chenopodium quinoa , biology , mottle , virus , differential centrifugation , chlorosis , density gradient , inoculation , sucrose , plant virus , chenopodiaceae , spots , seedling , dwarfing , botany , virology , horticulture , biochemistry , physics , rootstock , quantum mechanics
SUMMARY Strawberry mottle virus (SMoV) (three isolates: HJ, 3E and N) were transmitted to Chenopodium quinoa plants by sap inoculation. All three isolates induced very similar symptoms consisting of chlorotic spots and ringspots in inoculated leaves, and vein chlorosis, mottling, and dwarfing of the upper leaves. SMoV isolate HJ was purified from infected C. quinoa by homogenisation with 10 mM phosphate buffer, pH 7.2 containing 5% Triton X‐100, followed by differential, sucrose density‐gradient and CsCl equilibrium density‐gradient centrifugations. A fraction with a buoyant density of 1.42g ‐ cm ‐3 after CsCl density‐gradient centrifugation was highly infectious to C. quinoa and contained many isometric virus‐like particles c. 37 nm in diameter. These virus‐like particles were never found in fractions from uninfected preparations. Electrophoretic analysis of a fraction containing virus‐like particles revealed that these particles might have a single coat protein subunit with the apparent molecular mass of 26 K daltons and one nucleic acid of 6.6 kilobases. Double‐stranded RNA analysis of isolate HJ‐infected or uninfected C. quinoa and Fragaria vesca var. semperflorens seedling line ‘Alpine’ plants showed that both infected plants had two infection‐specific dsRNA bands of mol. wts 4.5 and 3.9 × 10 6 .