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Particle purification and properties of cassava Ivorian bacilliform virus
Author(s) -
FARGETTE D.,
ROBERTS I. M.,
HARRISON B. D.
Publication year - 1991
Publication title -
annals of applied biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.677
H-Index - 80
eISSN - 1744-7348
pISSN - 0003-4746
DOI - 10.1111/j.1744-7348.1991.tb04869.x
Subject(s) - biology , nicotiana benthamiana , virus , antiserum , virology , chenopodium quinoa , inoculation , plant virus , chenopodium , indicator plant , latent virus , host (biology) , potato virus x , botany , horticulture , antibody , ecology , immunology , weed
Summary A virus found in cassava from the north‐west of the Ivory Coast was transmitted by inoculation with sap extracts to herbaceous species in six plant families. Chenopodium quinoa was used as a propagation host and C. murale was used for local lesion assays. The virus particles are bacilliform, c. 18 nm in diameter, with predominant lengths of 42,49 and 76 nm and a structure apparently similar to that found in alfalfa mosaic virus. Purified preparations of virus particles had A 260 /A 280 of 1.7 ±0.05, contained one protein of M r c. 22 000, and yielded three species of RNA with M r (× 10 ‐6 ) of c. 0.7, 0.8 and 1.2. Although the virus particles were poorly immunogenic, an antiserum was produced and the virus was detected by enzyme‐linked immunosorbent assay (DAS‐ELISA) in leaf extracts at concentrations down to c. 6 ng/ml. Four other field isolates were also detected, including a strain which caused only mild systemic symptoms in C. quinoa instead of necrosis. The naturally infected cassava source plants were also infected with African cassava mosaic virus (ACMV) but when the new virus was cultured in Nicotiana benthamiana , either separately or together with ACMV, its concentration was the same. The new virus did not react with antisera to several plant viruses with small bacilliform or quasi‐bacilliform particles, and alfalfa mosaic virus reacted only weakly and inconsistently with antiserum to the cassava virus. The new virus, for which the name cassava Ivorian bacilliform virus is proposed, is tentatively classified as the second member of the alfalfa mosaic virus group.