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Detection of bean yellow mosaic virus in gladioli corms by the polymerase chain reaction
Author(s) -
VUNSH RON,
ROSNER ARIE,
STEIN ADINA
Publication year - 1991
Publication title -
annals of applied biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.677
H-Index - 80
eISSN - 1744-7348
pISSN - 0003-4746
DOI - 10.1111/j.1744-7348.1991.tb04867.x
Subject(s) - biology , polymerase chain reaction , corm , virology , virus , microbiology and biotechnology , rna , sephadex , biochemistry , botany , gene , enzyme
Summary The polymerase chain reaction (PCR) method readily detected bean yellow mosaic virus (BYMV) in gladioli leaves, but in initial tests PCR did not detect virus in corm tissue. Extracts of RN A from corm tissue were shown to inhibit the amplification of viral sequences when added to a PCR reaction. An additional purification step for the RNA extracts using a Sephadex G‐50 column eliminated the inhibitory effect and enabled PCR to amplify and detect viral RNA in corm tissue preparations.