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Screening for fire blight resistance in apple ( Malus pumila ) using excised leaf assays from in vitro and in vivo grown material
Author(s) -
DONOVAN ANNE
Publication year - 1991
Publication title -
annals of applied biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.677
H-Index - 80
eISSN - 1744-7348
pISSN - 0003-4746
DOI - 10.1111/j.1744-7348.1991.tb04843.x
Subject(s) - fire blight , biology , bioassay , blight , erwinia , malus , horticulture , cultivar , botany , bacteria , genetics
Summary The apple cultivars Greensleeves and Novole and somaclones derived from Greensleeves were tested for resistance to fire blight ( Erwinia amylovora ) using excised leaf bioassays. Cv. Greensleeves was consistently susceptible to fire blight in in vitro , and in glasshouse and excised leaf bioassays whilst cv. Novole was resistant. Greensleeves somaclones, identified as having intermediate levels of fire blight resistance in glasshouse and in vitro screening tests, also showed intermediate levels of resistance in excised leaf bioassays. The length of leaves and the inoculum concentration employed affected the severity of symptoms observed. Excised leaf bioassays were unsuitable as tests for fire blight resistance using field grown material.