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Characterisation of a new strain of tobacco necrosis virus isolated from nutrient feeding solution *
Author(s) -
ADAM G.,
WINTER S.,
LESEMANN D.E.
Publication year - 1990
Publication title -
annals of applied biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.677
H-Index - 80
eISSN - 1744-7348
pISSN - 0003-4746
DOI - 10.1111/j.1744-7348.1990.tb06635.x
Subject(s) - biology , ouchterlony double immunodiffusion , plant virus , antiserum , virology , rna , virus , strain (injury) , biochemistry , antibody , gene , immunology , anatomy
Summary A virus, isolated from the nutrient feeding solution of a bell pepper culture in a glasshouse, was characterised as a new serotype of tobacco necrosis necrovirus (TNV) and named TNV‐nft. The virus was monopartite and contained a single RNA species and a single coat protein with molecular weights of 1.45± 10 6 and 2.9 ± 10 4 daltons, respectively. The particle had an apparent sedimentation coefficient of 110 S and a density of 1.37 g/cm 3 in cesium chloride. In decoration tests antiserum prepared against TNV‐nft reacted most strongly with the TNV strains Kassanis A and B, but at lower dilutions it also reacted with all other TNV‐serotypes available. In Ouchterlony tests spur formation occurred with TNV strains Kassanis A and B, indicating that the TNV‐nft strain was serologically distinct. The isolate was not associated with a satellite virus. Cloned cDNA prepared against viral RNA reacted exclusively with the viral RNA band in Northern blots.