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Further properties of wineberry latent virus and evidence for its possible involvement in calico disease
Author(s) -
JONES A. T.,
MITCHELL M. J.,
McGAVIN W. J.,
ROBERTS I. M.
Publication year - 1990
Publication title -
annals of applied biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.677
H-Index - 80
eISSN - 1744-7348
pISSN - 0003-4746
DOI - 10.1111/j.1744-7348.1990.tb04823.x
Subject(s) - biology , closterovirus , virus , antiserum , virology , plant virus , rna , nucleic acid , botany , antibody , biochemistry , genetics , gene
Summary The flexuous filamentous particles of wineberry latent virus (WLV) were found to measure 620. 12 nm and not 510. 12 nm as previously reported. Analysis of dsRNA from infected plants detected a major species of c. 5.7. 10 6 mol. wt and minor species of lower mol. wt. Purified virus particles formed a major and a minor buoyant density component in solutions of caesium salts with densities of 1.26 and 1.25 g cm ‐3 in Cs 2 SO 4 and 1.30 and 1.29 g cm ‐3 in CsCl. The particles contained a single nucleic acid species, presumably single stranded RNA, and a single polypeptide of estimated mol. wt 2.78. 10 6 and 31 000 respectively. In indirect ELISA, purified particles of WLV and particles in plant sap failed to react specifically with antiserum to nine carlaviruses, 12 potexviruses, three capilloviruses or apple chlorotic leafspot closterovirus, nor was WLV found to react with several of these antisera in immunosorbent electron microscopy or immunoblots. In Marion and Olallie blackberry, WLV in mixture with raspberry bushy dwarf virus (RBDV), but not RBDV alone, induced veinal line‐pattern symptoms resembling those of calico disease reported from the USA.