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A ribosomal DNA probe to distinguish populations of Rhopalosiphum maidis (Homoptera: Aphididae)
Author(s) -
LUPOLI R.,
IRWIN M. E.,
VOSSBRINCK C. R.
Publication year - 1990
Publication title -
annals of applied biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.677
H-Index - 80
eISSN - 1744-7348
pISSN - 0003-4746
DOI - 10.1111/j.1744-7348.1990.tb04189.x
Subject(s) - biology , homoptera , aphididae , rhopalosiphum maidis , ribosomal dna , dna , mitochondrial dna , ribosomal rna , restriction enzyme , gel electrophoresis , botany , genetics , microbiology and biotechnology , pest analysis , gene , phylogenetics
SUMMARY Total DNA from clones of Rhopalosiphum maidis was digested with restriction endonucleases and hybridised with a heterospecific ribosomal DNA probe. DNA banding patterns exhibited sufficient differences to distinguish five different clones, and provided greater resolving power than gel electrophoresis of allozymes. The mechanisms and applications of this marking technique are discussed.