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Quantitative and qualitative aspects in the production of a nuclear polyhedrosis virus in Spodoptera exigua larvae
Author(s) -
SMITS P. H.,
VLAK J. M.
Publication year - 1988
Publication title -
annals of applied biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.677
H-Index - 80
eISSN - 1744-7348
pISSN - 0003-4746
DOI - 10.1111/j.1744-7348.1988.tb02061.x
Subject(s) - biology , exigua , nuclear polyhedrosis virus , spodoptera , larva , instar , virus , autographa californica , virology , botany , biochemistry , gene , recombinant dna
SUMMARY Spodoptera exigua nuclear polyhedrosis virus was produced in late fourth instar S. exigua larvae, reared on semi‐artificial diet. A maximum amount of virus, 1–2 × 10 9 polyhedra/larva, was produced in individually‐reared larvae after 7 days of incubation, with an inoculum of 7–5 × 10 4 polyhedra/cm 2 diet surface. Virus yield was slightly reduced to 9 × 10 8 polyhedra/larva when production was carried out in groups of 400 and 600 larvae per container. Biological activity of virus harvested from living larvae and from dead cadavers was similar. Microbial contaminants, predominantly bacteria, in the virus product numbered 1–6% of the number of polyhedra. Tests for the presence of vertebrate‐pathogenic bacteria in the virus product were all negative.