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Identification and characterisation of a newly described potyvirus in West Africa: Asystasia mottle virus
Author(s) -
THOUVENEL J.C.,
FAUQUET C.,
FARGETTE D.
Publication year - 1988
Publication title -
annals of applied biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.677
H-Index - 80
eISSN - 1744-7348
pISSN - 0003-4746
DOI - 10.1111/j.1744-7348.1988.tb02047.x
Subject(s) - biology , mottle , virology , virus , antiserum , potyvirus , titer , datura stramonium , aphis craccivora , plant virus , homoptera , botany , aphididae , antibody , immunology , pest analysis
SUMMARY Asystasia mottle virus (AsMV) was detected serologically in samples of Asystasia gangetica with mottle symptoms, from several areas of tropical West Africa. It infected 12 species systemically and induced local lesions in a further four. The virus lost infectivity after dilution to 10 ‐4 , after 10 min at 75 °C and after 3 days at 27°C. Purified virus had an A 260 / A 280 ratio of c. 1·2 and a protein subunit mol. wt of c. 33 000. Particles were c. 750 nm long and cytoplasmic inclusions typical of potyviruses were seen in ultrathin sections of infected leaves. The antiserum prepared had a titre of 1/1024 in microprecipitin tests but purified virus failed to react with 31 antisera to known potyviruses. The virus was transmitted in the non‐persistent manner by Aphis spiraecola but only very infrequently. On the basis of these properties, AsMV is considered to be a new member of the potyvirus group.