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Use of monoclonal antibody to potato leafroll virus for detecting groundnut rosette assistor virus by ELISA *
Author(s) -
RAJESHWARI R.,
MURANT A. F.,
MASSALSKI THE LATE P. R.
Publication year - 1987
Publication title -
annals of applied biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.677
H-Index - 80
eISSN - 1744-7348
pISSN - 0003-4746
DOI - 10.1111/j.1744-7348.1987.tb01462.x
Subject(s) - luteovirus , potato leafroll virus , biology , aphis craccivora , canavalia ensiformis , rosette (schizont appearance) , virology , plant virus , monoclonal antibody , aphididae , virus , botany , antibody , agronomy , homoptera , pest analysis , immunology
SUMMARY Three of 10 monoclonal antibodies (MAbs) produced to potato leafroll luteovirus (PLRV) were found to react in triple antibody sandwich ELISA (TAS‐ELISA) with groundnut rosette assistor luteovirus (GRAV), though none reacted with four other luteoviruses (barley yellow dwarf, bean leaf roll, beet western yellows or carrot red leaf)‐ The most effective PLRV MAb, SCR 6, was used in TAS‐ELISA to detect isolates of GRAV from groundnut plants with chlorotic, green and mosaic forms of rosette from Nigeria and Malawi. The test also detected GRAV in extracts of single Aphis craccivora.