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Purification and partial characterisation of beet mild yellowing virus and its serological detection in plants and aphids
Author(s) -
GOVIER D. A.
Publication year - 1985
Publication title -
annals of applied biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.677
H-Index - 80
eISSN - 1744-7348
pISSN - 0003-4746
DOI - 10.1111/j.1744-7348.1985.tb03160.x
Subject(s) - aphid , biology , immunodiffusion , virus , antiserum , serology , extraction (chemistry) , titer , chromatography , virology , botany , antigen , chemistry , antibody , genetics , immunology
SUMMARY Beet mild yellowing virus (BMW) was reversibly precipitated at temperatures below about 5°C and this property was used as a final step in a purification procedure which yielded about 1 mg virus/kg tissue. Purified virus was infective and had an A 200/ A 280 ratio of about 1–8. BMW particles were isometric with a diameter of 26 nm, sedimented at 116 S , had a buoyant density in caesium chloride of 1.42 g/cm 3 and a coat protein mol. wt of 25 400. An antiserum to BMW had a titre in immunodiffusion tests of 1/256 and was used in immunodiffusion tests, immunospecific electron microscopy (ISEM) and enzyme‐linked immunosorbent assay to demonstrate a close serological relationship between BMW and beet western yellows virus. BMW was readily detected by ISEM in plants and also in aphid vectors after treatment of aphid extracts with a chloroform:butanol mixture.