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Inactivation of a nuclear polyhedrosis virus on cotton by the substances produced by the cotton leaf surface glands
Author(s) -
ELLEMAN C. J.,
ENTWISTLE P. F.
Publication year - 1985
Publication title -
annals of applied biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.677
H-Index - 80
eISSN - 1744-7348
pISSN - 0003-4746
DOI - 10.1111/j.1744-7348.1985.tb03097.x
Subject(s) - nuclear polyhedrosis virus , biology , spodoptera littoralis , noctuidae , exudate , virus , calcium , horticulture , botany , agronomy , lepidoptera genitalia , virology , chemistry , organic chemistry
SUMMARY Loss of viability of a nuclear polyhedrosis virus (NPV) of Spodoptera littoralis was shown to occur on cotton leaves but not on cabbage leaves or on semi‐synthetic insect diet. The inactivation was rapid, occurred in the dark and required contact between the cotton leaf surface and the polyhedral inclusion bodies (PIBs). It was shown that the products of the cotton leaf glands, which exude a crystalline material onto the leaf surface, could account for the rapid inactivation observed. Inactivation did not involve loss of virus polyhedral inclusion bodies. The use of EDTA or a low pH buffer in the NPV suspension reduced the inactivation which suggested that the loss of activity was due to the presence of calcium and magnesium in the cotton gland exudate.