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Peanut stripe virus ‐ a new seed‐borne potyvirus from China infecting groundnut ( Arachis hypogaea ) 1
Author(s) -
DEMSKI J. W.,
REDDY D. V. R.,
SOWELL G.,
BAYS D.
Publication year - 1984
Publication title -
annals of applied biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.677
H-Index - 80
eISSN - 1744-7348
pISSN - 0003-4746
DOI - 10.1111/j.1744-7348.1984.tb03075.x
Subject(s) - biology , arachis hypogaea , mottle , potyvirus , plant virus , aphis craccivora , chenopodium , hemagglutinin (influenza) , inoculation , mosaic virus , potyviridae , arachis , virus , alfalfa mosaic virus , tobacco mosaic virus , botany , virology , horticulture , aphididae , homoptera , rna , coat protein , biochemistry , gene , pest analysis , weed
SUMMARY A new virus, peanut stripe (PStV), isolated from groundnut ( Arachis hypogaea ) in the USA, induced characteristic striping, discontinuous vein banding along the lateral veins, and oakleaf mosaic in groundnut. The virus was also isolated from germplasm lines introduced from the People's Republic of China. PStV was transmitted by inoculation of sap to nine species of the Chenopodiaceae, Leguminosae, and Solanaceae; Chenopodium amaranticolor was a good local lesion host. PStV was also transmitted by Aphis craccivora in a non‐persistent manner and through seed of groundnut up to 37%. The virus remained infective in buffered plant extracts after diluting to 10 ‐3 , storage for 3 days at 20°C, and heating for 10 min at 60°C but not 65°C. Purified virus preparations contained flexuous filamentous particles c. 752 nm long, which contained a major polypeptide of 33 500 daltons and one nucleic acid species of 3·1 × 10 6 daltons. In ELISA, PStV was serologically related to blackeye cowpea mosaic, soybean mosaic, clover yellow vein, and pepper veinal mottle viruses but not to peanut mottle, potato Y, tobacco etch, and peanut green mosaic viruses. On the basis of these properties PStV is identified as a new potyvirus in groundnut.