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Immunoenzymatic techniques for the detection of Phoma exigua in infected potato tissues
Author(s) -
AGUELON M.,
DUNEZ J.
Publication year - 1984
Publication title -
annals of applied biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.677
H-Index - 80
eISSN - 1744-7348
pISSN - 0003-4746
DOI - 10.1111/j.1744-7348.1984.tb03072.x
Subject(s) - biology , exigua , antiserum , phoma , mycelium , fungus , inoculation , monoclonal antibody , antibody , microbiology and biotechnology , fungi imperfecti , botany , horticulture , spodoptera , biochemistry , immunology , recombinant dna , gene
SUMMARY Antisera to Phoma exigua var. foveata and var. exigua were prepared by injecting rabbits and mice with protein solutions from mycelium. Specific antibodies were isolated and immunoenzymatic techniques (double antibody sandwich ELISA and indirect ELISA) were used to test for the fungus in inoculated tubers and sprouts and in stems grown from these tubers. The fungus was detected in these different tissues, with var. foveata being more aggressive, demonstrating the applicability and sensitivity of the techniques. The antibodies produced to the two varieties of the fungus were not specific to their own varieties. They also reacted with Phoma tracheiphila but did not react with several other common potato pathogens. Preliminary results obtained with antibodies from mouse ascite liquid suggest the possibility of producing specific monoclonal antibodies.