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Screening for resistance to potato cyst nematodes using bioluminescent photometry
Author(s) -
STOREY R. M. J.,
MARKS R. J.
Publication year - 1983
Publication title -
annals of applied biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.677
H-Index - 80
eISSN - 1744-7348
pISSN - 0003-4746
DOI - 10.1111/j.1744-7348.1983.tb02770.x
Subject(s) - biology , globodera pallida , cyst , potato cyst nematode , globodera rostochiensis , photometry (optics) , nematode , veterinary medicine , botany , horticulture , zoology , ecology , genetics , solanaceae , gene , physics , radiology , medicine , stars , astronomy
SUMMARY The measurement of the adenosine triphosphate (ATP) content of cysts by bioluminescent photometry has been used as a screen for assessing the resistance of five potato clones to Globodera rostochiensis Rol and G. pallida Pa3. The multiplication rates (P f /P i ) of the nematodes are based on the ratio of ATP levels for the cyst inoculum and their progeny. These have been compared to the corresponding ratios based on conventional visual counts of eggs and juveniles. The use of recoverable standard volumes of cysts to inoculate pots has allowed an assessment of the unhatched proportion (C p ) of juveniles from the parental cysts by both methods and an estimate of the corrected multiplication rate (P/P^l‐Cp)). This is an accurate assessment of resistance for a clone and the use of standard inocula and the ATP method will enable the criterion adopted for establishing resistance to be measured rapidly and precisely.