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The use of F(ab‘) 2 ‐based ELISA to detect serological relationships among carlaviruses
Author(s) -
ADAMS A. N.,
BARBARA D. J.
Publication year - 1982
Publication title -
annals of applied biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.677
H-Index - 80
eISSN - 1744-7348
pISSN - 0003-4746
DOI - 10.1111/j.1744-7348.1982.tb00850.x
Subject(s) - biology , antiserum , serology , virology , latent virus , virus , hop (telecommunications) , antibody , genetics , computer network , computer science
SUMMARY An indirect, F(ab) 2 ‐based ELISA was used to assess the reactions of seven carlaviruses with antisera to 16 viruses within the group and with antisera to seven other filamentous viruses. There were no specific reactions with antisera to non‐carlaviruses and serological relationships between the seven carlaviruses were generally consistent with results reported by other workers using different methods. Thus, hop latent, hop mosaic, carnation latent, potato virus S and lily symptomless viruses seem antigenically similar while American hop latent and poplar mosaic viruses are distinct from these viruses and from each other. The F(ab) 2 ‐based method is simple and rapid and it is particularly useful for probing relationships when only small quantities of antisera are available.