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Chemical suppression of virus in cultured plant tissues
Author(s) -
SIMPKINS I.,
WALKEY D. G. A.,
NEELY HEATHER A.
Publication year - 1981
Publication title -
annals of applied biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.677
H-Index - 80
eISSN - 1744-7348
pISSN - 0003-4746
DOI - 10.1111/j.1744-7348.1981.tb05143.x
Subject(s) - biology , kinetin , ribavirin , incubation , virus , virology , cucumber mosaic virus , meristem , tissue culture , botany , plant virus , horticulture , in vitro , biochemistry , shoot , hepatitis c virus
SUMMARY The concentrations of cucumber mosaic (CMV) and alfalfa mosaic viruses, present in various types of cultured plant tissues, were significantly less when ribavirin (syn. Virazole) at 50 or 100 mg/litre was present in the culture medium. CMV concentrations decreased within 24 days in infected cultures treated with ribabirin, but incubation periods of up to 127 days were required before the virus was undetectable. However, virus‐free cultures were obtained from CMV‐infected meristem‐tips irrespective of the presence or absence of ribavirin. Kinetin at concentrations up to 25.6 mg/litre had no persistent antiviral effect.