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Methods for detection of resistance to woolly aphid ( Eriosoma lanigerum ) in apple
Author(s) -
LYTH M.,
WATKINS R.
Publication year - 1981
Publication title -
annals of applied biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.677
H-Index - 80
eISSN - 1744-7348
pISSN - 0003-4746
DOI - 10.1111/j.1744-7348.1981.tb00420.x
Subject(s) - biology , inoculation , aphid , nymph , horticulture , instar , homoptera , resistance (ecology) , botany , pest analysis , agronomy , larva
SUMMARY Two ways of examining resistance in apple to Eriosoma lanigerum were assessed. The first method involved a single mass inoculation of young seedlings with first‐instar nymphs. This failed to identify many susceptible seedlings and also produced inconsistent results which strongly suggested an uneven distribution of aphids at inoculation. Repeated inoculations were far more efficient in identifying known susceptibles than single inoculations, but they did not discriminate between susceptible and some resistant seedlings. However, the technique would be very useful where the major objective was the identification of higher levels of resistance. To overcome the destructive nature of mass inoculation and the inefficiency in classifying individual seedlings, a second method was devised using clonal material which allowed replication of genotypes. Calibrated inoculations made direct comparisons with controls possible and aphid numbers could be estimated with acceptable accuracy with a volumetric technique. However, comparisons based on approximate colony lengths were adequate, and faster, for routine screening.