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Hibiscus latent ringspot virus, a newly recognised virus from Hibiscus rosa‐sinensis (Malvaceae) in western Nigeria
Author(s) -
BRUNT A. A.,
BARTON R. J.,
PHILLIPS SUE,
LANA A. O.
Publication year - 1980
Publication title -
annals of applied biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.677
H-Index - 80
eISSN - 1744-7348
pISSN - 0003-4746
DOI - 10.1111/j.1744-7348.1980.tb04766.x
Subject(s) - hibiscus , biology , nepovirus , malvaceae , virus , chenopodium , plant virus , botany , virology , weed
SUMMARY Hibiscus latent ringspot virus (HLRV) was prevalent in Hibiscus rosa‐sinensis in Ibadan, Nigeria. It was readily transmitted mechanically to 22 of 73 species from seven of 20 families, but was best propagated in Nicotiana clevelandii or Hibiscus cannabinus and assayed in Chenopodium murale . HLRV was readily purified from systemically infected hosts by differential centrifugation of leaf extracts clarified with 8.0% n‐butanol, followed by molecular permeation chromatography on controlled‐pore glass beads (700 Å, 120–200 mesh). The virus has isometric particles c . 28 nm in diameter which sedimented as three components (T, M and B), with sedimentation coefficients (s° 20, w ) of 51; 114 and 132 S and buoyant densities in caesium chloride of 1.32, 1.49 and 1.52 g/cm 3 , respectively. All three components contained a single polypeptide of rnol. wt 53.6 × 10 3 . T component particles contained only protein but M and B components also contained single‐stranded RNA of rnol. wt 2.2 × 10 6 and 2.5 × 10 6 , respectively. The properties of HLRV suggest affinities with nepoviruses but no serological relationship was detected between HLRV and 15 recognised or possible members of the nepovirus group.