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A freeze/thaw technique for estimation of nitrogenase activity in detached nodules of Vigna mungo
Author(s) -
SRIVASTAVA R. C.,
MUKERJI D.,
MATHUR S. N.
Publication year - 1980
Publication title -
annals of applied biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.677
H-Index - 80
eISSN - 1744-7348
pISSN - 0003-4746
DOI - 10.1111/j.1744-7348.1980.tb02984.x
Subject(s) - nitrogenase , vigna , biology , rhizobia , root nodule , nodule (geology) , nitrogen fixation , toluene , enzyme assay , botany , enzyme , horticulture , biochemistry , chemistry , bacteria , paleontology , organic chemistry , genetics
SUMMARY Freezing (under inert conditions) of detached root nodules (with active rhizobia) in the presence of 0.05 ml toluene followed by thawing allowed N 2 ‐dependent evolution of ammonia. The activity of the frozen/thawed and toluene‐treated nodules was affected similarly to that expected of nitrogenase by various known effectors of the enzyme. This freezehhaw technique thus offers an alternative method for estimation of nitrogenase activity in whole nodule preparations.