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The elimination of cocksfoot streak virus, cocksfoot mild mosaic virus and cocksfoot mottle virus from Dactylis glomerata by shoot tip and tiller bud culture
Author(s) -
DALE PHILIP J.
Publication year - 1979
Publication title -
annals of applied biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.677
H-Index - 80
eISSN - 1744-7348
pISSN - 0003-4746
DOI - 10.1111/j.1744-7348.1979.tb06543.x
Subject(s) - biology , dactylis glomerata , tiller (botany) , shoot , barley yellow dwarf , virus , botany , mottle , plant virus , poaceae , virology
SUMMARY Shoot tips from 0.2 to 4.8 mm long and tiller buds 0.2 to 5.1 mm long were cultured from cocksfoot ( Dactylis glomerata ) plants known to be infected with combinations of cocksfoot streak, cocksfoot mild mosaic and cocksfoot mottle viruses. Regenerated plants were tested for the presence of viruses by electron microscopy, serology and the expression of symptoms. All viruses were eliminated by culturing shoot tips and tiller buds less than 1.2 mm long but the maximum explant size capable of regenerating healthy plants depended upon the infecting virus. Viruses were not detected in shoot tip and tiller bud samples of similar size to the explants that gave healthy plants. The method is of value in eliminating viruses from desirable stocks of D. glomerata that must be vegetatively propagated.