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The relationship of potato black ringspot virus to tobacco ringspot and allied viruses
Author(s) -
SALAZAR L. F.,
HARRISON B. D.
Publication year - 1978
Publication title -
annals of applied biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.677
H-Index - 80
eISSN - 1744-7348
pISSN - 0003-4746
DOI - 10.1111/j.1744-7348.1978.tb02647.x
Subject(s) - biology , nepovirus , tobacco mosaic virus , rna , virology , tobacco etch virus , plant virus , infectivity , virus , genetics , potyvirus , gene
SUMMARY The relationship between potato black ringspot virus (PBRV), isolates of tobacco ringspot virus from blueberry (TRSV‐B), cherry (TRSV‐C) and calico‐diseased potato (TRSV‐P), and eucharis mottle virus (EuMV) was examined in tests of three types. In gel‐diffusion precipitin tests, the reaction end‐points of antisera, and spur formation, indicated that PBRV and TRSV‐P are very closely related but not identical antigenically, as are TRSV‐B and TRSV‐C, and that these two pairs are more distantly related to each other and to EuMV. In plant‐protection tests in Nicotiana angustifolia , PBRV, TRSV‐B and EuMV conferred protection against the homologous virus but not against one another. PBRV, but not TRSV‐B, conferred protection against TRSV‐P. In tests with the two RNA species of PBRV, infectivity increased greatly when preparations of RNA‐1 and RNA‐2 were mixed, and both species are probably needed for infection. Infectivity did not increase when RNA‐1 or RNA‐2 of PBRV was mixed with RNA‐2 or RNA‐1, respectively, of TRSV‐B; the two viruses seem too distantly related to form pseudo‐recombinants. It is concluded that PBRV and tobacco ringspot virus should be considered separate viruses, and that TRSV‐P should be considered a strain of PBRV. EuMV should perhaps be recognised as a virus distinct from, but related to, PBRV and tobacco ringspot virus.