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The use of enzyme‐linked immunosorbent assay to detect, and discriminate between, small iridescent viruses
Author(s) -
KELLY D. C.,
EDWARDS MARYL.,
ROBERTSON J. S.
Publication year - 1978
Publication title -
annals of applied biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.677
H-Index - 80
eISSN - 1744-7348
pISSN - 0003-4746
DOI - 10.1111/j.1744-7348.1978.tb02645.x
Subject(s) - biology , galleria mellonella , iridescence , virus , virology , enzyme , antibody , microbiology and biotechnology , immunology , virulence , biochemistry , ecology , gene
SUMMARY The enzyme‐linked immunosorbent assay (ELISA) double antibody method provided an efficient method for detecting iridescent virus (type 22) in purified preparations and extracts of Galleria mellonella larvae; 10 ng of purified virus/ml were detected with confidence. The ELISA method discriminated between the five iridescent viruses tested.