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Thermal inactivation of cherry leaf roll virus in tissue cultures of Nicotiana rustica raised from seeds and meristem‐tips
Author(s) -
COOPER VALERIE C.,
WALKEY D. G. A.
Publication year - 1978
Publication title -
annals of applied biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.677
H-Index - 80
eISSN - 1744-7348
pISSN - 0003-4746
DOI - 10.1111/j.1744-7348.1978.tb00706.x
Subject(s) - biology , infectivity , meristem , incubation , botany , nicotiana , solanaceae , virus , tissue culture , horticulture , virology , shoot , in vitro , biochemistry , gene
SUMMARY Nicotiana rustica tissue cultures derived from seeds or embryos infected with cherry leaf roll virus (CLRV), remained infected after culture at 22 o C. No infectivity was found in cultures held at 32 o C for 5 days but it was readily detected after such cultures were transferred to 25 o C for 8 days. Virus was permanently eradicated from most plants after 20 days incubation at 32 o C and from all plants after 7 days incubation at 40 o C. Partially purified preparations of CLRV lost infectivity after 9–12 days at 22 o C, 5 days at 32 o C and 3 days at 40 o C.