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Partial purification and some properties of wineberry latent, a virus obtained from Rubus phoenicolasius
Author(s) -
JONES A. T.
Publication year - 1977
Publication title -
annals of applied biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.677
H-Index - 80
eISSN - 1744-7348
pISSN - 0003-4746
DOI - 10.1111/j.1744-7348.1977.tb01832.x
Subject(s) - rubus , biology , chenopodium quinoa , infectivity , sucrose , herbaceous plant , peg ratio , virus , inoculation , desiccation , botany , ultracentrifuge , chenopodium , polyethylene glycol , horticulture , food science , virology , biochemistry , finance , economics , weed
SUMMARY Wineberry latent virus (WLV) was obtained from a single symptomless plant of American wineberry ( Rubus phoenicolasius ) originally imported from the United States of America. On graft inoculation, WLV infected but induced no distinctive symptoms in several Rubus species including those used as indicators for known Rubus viruses. It was not seed‐borne in wineberry. WLV was mechanically transmitted to several herbaceous species but induced local lesions in only a few; it was weakly systemic in some Chenopodium species. Infective C. quinoa sap lost infectivity after diluting to 10 ‐4 , heating for 10 min at 70°C, and storage either for 8 days at 18°C or for 32 days at 4°C. Sap from infected plants contained flexuous filamentous particles c. 510°12 nm. WLV was partially purified by extracting infected C. quinoa leaves in 0·05 M tris‐HCl buffer (pH 7 ) containing 0·2% thio‐glycerol and 10% (v/v) chloroform and concentrating virus by precipitation with 7% (w/v) polyethylene glycol (PEG, mol. wt 6000) and 0·1 NaCl. The virus was then pelleted through a 30% (w/v) sucrose pad containing 7% PEG+0·1 M NaCl and finally sedimented through a sucrose density‐gradient. These preparations had A 260/280 ratios of 1·26, contained end to end aggregates of WLV particles and formed a partly polydispersed peak in the analytical ultracentrifuge. WLV did not react with antisera to four potex‐viruses, or to apple chlorotic leaf spot or apple stem grooving viruses.