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Benomyl tolerance in Verticillium dahliae
Author(s) -
TALBOYS P. W.,
DAVIES MOIRA K.
Publication year - 1976
Publication title -
annals of applied biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.677
H-Index - 80
eISSN - 1744-7348
pISSN - 0003-4746
DOI - 10.1111/j.1744-7348.1976.tb01670.x
Subject(s) - benomyl , mycelium , biology , conidium , fungicide , verticillium dahliae , verticillium , benzimidazole , botany , verticillium wilt , horticulture , chemistry , organic chemistry
SUMMARY In Verticillium dahliae isolates (‘wild type’) obtained prior to the advent of benzimidazole fungicides, growth of germ tubes and extension of mycelium were typically inhibited by benomyl at 0–3 ppm and 0.5‐1.0 ppm respectively. Culture of such isolates in the presence of increasing concentrations of benomyl resulted in the selection of variants able to grow at 12.0 ppm. Conidia, and mycelium grown for 24 b, were each more sensitive to inhibition by benomyl than mycelium grown for 48 h. Mycelium of both wild‐type and selected variants was still viable after treatment with 20 ppm benomyl in a nutrient medium for 112 days. Long‐term treatments at 20 ppm caused the enlargement of mycelial cells and conidia, thickening of the cell walls and formation of large spherical inclusions apparently comprising or containing lipids.