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Effect of temperature on virus eradication and growth of infected tissue cultures
Author(s) -
WALKEY D. G. A.,
COOPER VALERIE C.
Publication year - 1975
Publication title -
annals of applied biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.677
H-Index - 80
eISSN - 1744-7348
pISSN - 0003-4746
DOI - 10.1111/j.1744-7348.1975.tb01620.x
Subject(s) - biology , virology , virus , tissue culture , microbiology and biotechnology , genetics , in vitro
SUMMARY Cucumber mosaic (CMV) and alfalfa mosaic (AlfMV) viruses were eradicated or their concentrations greatly diminished when infected meristem tips were grown in static or shake culture at 32 or 34 o C. Similar cultures grown at 22 o C remained infected. Tobacco mosaic virus (TMV) was not eradicated from shake cultures at 32 o C and the concentration of TMV in such cultures at 32 o C was sometimes higher than at 22 o C. The concentration of CMV and AlfMV was considerably less in cultured tissues than in infected leaf material, but that of TMV was similar. Cultures infected with CMV or TMV grew more slowly than healthy cultures. Growth of AlfMV infected and healthy cultures was similar.