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Effect of additives and storage conditions on antibody titres of tobacco mosaic and southern bean mosaic virus antisera
Author(s) -
WATERWORTH H. E.,
LAWSON R. H.,
BLIZZARD J. W.
Publication year - 1973
Publication title -
annals of applied biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.677
H-Index - 80
eISSN - 1744-7348
pISSN - 0003-4746
DOI - 10.1111/j.1744-7348.1973.tb07738.x
Subject(s) - antiserum , precipitin , tobacco mosaic virus , biology , virus , sodium azide , virology , serology , food science , antibody , biochemistry , immunology
SUMMARY The effect on serological activity of methods of storing antisera to southern bean mosaic virus and tobacco mosaic virus was tested. Aliquots (0.5 ml) were (1) stored at —70, —20, +4, +26 or +37 °C, (2) repeatedly frozen and thawed, (3) stored in 5% peptone, 0.02% sodium azide or 50% glycerol, (4) diluted before storage, or (5) freeze‐dried. All samples were titrated against purified virus after 1, 6 and 12 months. Most of the treatments had little effect on the precipitin titres of the antisera, but the titres were decreased by storage at 37 °C. Mention of specific equipment, trade products or commercial companies does not constitute their endorsement by the U.S. Department of Agriculture over similar products or companies not mentioned.