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Attempts to define and mimic the effects of pollen on the development of lesions caused by Phoma betae inoculated onto sugarbeet leaves
Author(s) -
WARREN R. C.
Publication year - 1972
Publication title -
annals of applied biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.677
H-Index - 80
eISSN - 1744-7348
pISSN - 0003-4746
DOI - 10.1111/j.1744-7348.1972.tb05082.x
Subject(s) - biology , pollen , conidium , botany , inoculation , germ tube , agar , hypha , orange (colour) , horticulture , bacteria , genetics
SUMMARY Expanding lesions resulted when conidia of Phoma betae Frank, mixed with rye pollen, were inoculated on to sugarbeet leaves by a standard technique. Conidia without pollen generally caused non‐expanding necrotic spots; these could be made to spread later by covering them with pollen or orange juice, but not with water. Germ‐tube growth was quicker on water agar than on sugarbeet leaves. Pollen extract stimulated germ‐tube growth on leaves 10 h after inoculation and resulted in the production of knots of hyphae overlying areas where intercellular hyphae could be discerned and where expanding lesions developed. Some inorganic salts mimicked the stimulatory effect of pollen on germtube growth on agar slides, but only a mixture of hexose sugars with boric acid reproduced the effect of pollen on both numbers and size of expanding lesions caused by P. betae on sugarbeet leaves, and numbers of expanding lesions caused by Botrytis cinerea Pers. ex Fr. on bean leaves.