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Stabilization, culture and some properties of tulip halo necrosis virus
Author(s) -
MOWAT W. P.
Publication year - 1971
Publication title -
annals of applied biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.677
H-Index - 80
eISSN - 1744-7348
pISSN - 0003-4746
DOI - 10.1111/j.1744-7348.1971.tb04667.x
Subject(s) - infectivity , biology , inoculation , myzus persicae , aphid , virus , chenopodium , chenopodium quinoa , necrosis , botany , horticulture , virology , genetics , weed
SUMMARY Tulip halo necrosis virus, obtained from tulips with leaf necrosis, is very labile in crude sap but can be transmitted consistently by inoculating Nicotiana clevelandii plants with extracts made in pH 8 phosphate buffer containing a stabilizing agent such as 0.2M 2‐mercaptoethanol or 0.01M dithiothreitol. Of the fifteen species in five families of Angiosperms infected by inoculation with sap, few are suitable as sources of inoculum. Cultures of the virus can be maintained in Nicotiana clevelandii kept at 14 or 18d̀C but not at 21d̀C. Infectivity can be assayed in Chenopodium quinoa , in which necrotic local lesions are produced. Stabilized extracts of leaves were infective at a dilution of 1/16 but rarely at 1/32, and infectivity decreased disproportionately with dilution. Infectivity of all extracts was abolished in 10 min at 50d̀C and of some at 45d̀C, but survived when extracts were clarified using diethyl ether or trichlorotrifluoroethane. The virus was not transmitted by the aphid Myzus persicae .