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The long‐term survival of some plant viruses preserved by lyophilization
Author(s) -
HOLLINGS M.,
STONE OLWEN M.
Publication year - 1970
Publication title -
annals of applied biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.677
H-Index - 80
eISSN - 1744-7348
pISSN - 0003-4746
DOI - 10.1111/j.1744-7348.1970.tb05509.x
Subject(s) - biology , ampoule , freeze drying , phenol , host (biology) , virology , chromatography , ecology , chemistry , organic chemistry
SUMMARY Seventy‐four viruses were tested for survival after lyophilization of infective sap and subsequent storage in vacuum‐sealed ampoules at room temperature. Fifty‐seven viruses survived at least i year and 19 of them over 10 years. The best results were obtained from host plants not containing inhibitors of infection or potent phenol oxidase systems. Purified preparations were also lyophilized. Lyophilization offers many advantages for maintaining a culture collection of sap‐transmissible viruses.