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Bacteriophage and serological methods for the identification of Pseudomonas phaseolicola (Burkh.) Dowson
Author(s) -
TAYLOR J. D.
Publication year - 1970
Publication title -
annals of applied biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.677
H-Index - 80
eISSN - 1744-7348
pISSN - 0003-4746
DOI - 10.1111/j.1744-7348.1970.tb04618.x
Subject(s) - biology , serology , pseudomonas , bacteriophage , microbiology and biotechnology , antiserum , pseudomonadaceae , lysis , antigen , bacteria , antibody , escherichia coli , gene , immunology , biochemistry , genetics
SUMMARY Rapid phage and serological methods were compared with biochemical and bean‐pod inoculation tests for the identification of Pseudomonas phaseolicola . Phages II P, 12 P and 48 P were highly specific and did not lyse any of forty‐five other isolates from bean or the sixteen species of Pseudomonas tested. They were also, however, unable to lyse the rough colony forms of P. phaseolicola which were occasionally isolated. Phage 12 S lysed a wide range of Pseudomonas spp., including a proportion of P. phaseolicola isolates, the majority of which were race 2. Serological tests showed that the heat‐labile antigen possessed by P. phaseolicola was common to twelve other species of plant pathogenic pseudomonads. The heat‐stable antigen was more specific and was detected only in P. mors‐prunorum and P. primulae , neither of which occurs on bean. The two races of P. phaseolicola were indistinguishable in serological tests. It was concluded that both phage and antiserum tests provide specific, rapid and easily applied methods for the routine identification of P. phaseolicola isolates from plant and seed material.