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Methods of isolating the microflora of moulding, high ‐moisture barley in partially sealed silos
Author(s) -
MULINGE S. K.,
CHESTERS C. G. C.
Publication year - 1970
Publication title -
annals of applied biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.677
H-Index - 80
eISSN - 1744-7348
pISSN - 0003-4746
DOI - 10.1111/j.1744-7348.1970.tb04589.x
Subject(s) - sodium hypochlorite , husk , agar , moisture , hypochlorite , biology , food science , agar plate , whole grains , information silo , horticulture , materials science , agronomy , botany , chemistry , bacteria , composite material , genetics , organic chemistry , silo
SUMMARY Fungi were isolated on agar from barley stored at 18–44% moisture content in partially sealed silos by plating out whole, untreated grain as removed from the silos; surface‐washed whole grain; surface‐washed and then sodium hypochlorite‐sterilized whole grain; hypochlorite surface‐sterilized, dehusked grains cut vertically and plated with cut surfaces to the agar; husks washed several times after removal and plated as small groups. 5 mg aureomycin/100 ml agar was used to suppress bacterial growth. These methods are compared with others in common use. The isolated species have been tabulated to illustrate the various combinations occurring in grains and in aggregates of husks.