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Some properties of clover yellow vein, a virus from Trifolium repens L.
Author(s) -
HOLLINGS M.,
NARIANI T. K.
Publication year - 1965
Publication title -
annals of applied biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.677
H-Index - 80
eISSN - 1744-7348
pISSN - 0003-4746
DOI - 10.1111/j.1744-7348.1965.tb01219.x
Subject(s) - biology , acyrthosiphon pisum , virus , trifolium repens , aphid , aphis , aphis gossypii , homoptera , myzus persicae , virology , chenopodium , botany , horticulture , aphididae , pest analysis , weed
SUMMARY A virus differing from those previously described from leguminous plants was obtained from Trifolium repens in Surrey, and named clover yellow vein virus. It was transmitted by Myzus persicae and Acyrthosiphon pisum (not by Aphis fabae ) in the non‐persistent manner, but not through seed. The virus infected nineteen out of sixty‐one plant species inoculated with sap: it was cultured in Nicotiana clevelandii , which reacted diagnostically and developed intracellular inclusions, and assayed in N. tabacum or Chenopodium quinoa. N. clevelandii sap was still infective after dilution to 1/10,000, heating for 10 min. at 55d̀ C. but not 60° C., and storing for 8 days at 18° C. or 75 days at 0° C. Infectivity, but not serological activity, was abolished by 0.2 or 2.0 % formaldehyde. Symptoms of clover yellow vein virus (CIYVV) were suppressed, and the virus was not detected, in infected N. clevelandii plants grown for 4 weeks at 38° C. Symptoms and detectable virus returned 5–6 weeks after heat‐treatment ceased. The virus had particles of normal length 670 mμ, and was partially purified and concentrated by several methods. Virus preparations gave specific zones when centrifuged in density gradients. CIYVV shares many properties with the aphid‐transmitted, slightly flexible rod‐shaped viruses in group 8 of Brandes & Wetter, but no relationship was detected serologically to those tested or to four other viruses with particles of similar dimensions.