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Factors influencing seedling infection of barley by Ustilago nuda (Jens.) Rostr
Author(s) -
KAVANAGH T.
Publication year - 1964
Publication title -
annals of applied biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.677
H-Index - 80
eISSN - 1744-7348
pISSN - 0003-4746
DOI - 10.1111/j.1744-7348.1964.tb01186.x
Subject(s) - biology , coleoptile , seedling , inoculation , chlamydospore , spore , botany , ustilago , horticulture , biochemistry , gene
SUMMARY In the seedling method of inoculation of barley with Ustilago nuda , a chlamydospore suspension is introduced into decapitated coleoptiles held under partial vacuum. The optimum conditions for infection were provided by the use of a partial vacuum of 25 in. of mercury for 2 min. on coleoptiles 1‐1·5 cm. long: lower infection percentages resulted when the coleoptiles were over 1·5 cm. No benefit was obtained by suspending the spores in dextrose or peptone solutions. The variety Jet remained immune to infection.