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A quantitative laboratory method for inoculating cabbage seedlings with Plasmodiophora brassicae Woron
Author(s) -
CHAN A. G.,
FLINT ANNE E.,
HINTON R. A. L.
Publication year - 1964
Publication title -
annals of applied biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.677
H-Index - 80
eISSN - 1744-7348
pISSN - 0003-4746
DOI - 10.1111/j.1744-7348.1964.tb01171.x
Subject(s) - spore , biology , clubroot , inoculation , germination , fungus , botany , horticulture , nutrient , brassica , ecology
SUMMARY In studies on root‐hair and rhizodermal cell infection of cabbage seedlings by Plasmodiophora brassicae Woron. seeds were germinated in an incubator at 25°C. on nylon mesh circles placed on glass dishes containing nutrient solution. After 3 days the original nutrient solution was replaced by a similar one containing a known quantity of resting spores of the fungus. After a further 5–7 days in the incubator the roots were removed and stained. The infected plants were counted and the extent of infection on each plant was assessed in grades 0–4. Optimal infection was obtained by using spores from decomposing clubbed roots, by thoroughly washing the resting spores, and by using thirty plants per dish grown from seed of 1·5‐2·0 mm. diam. There was a rapid increase in infection from the 3rd to the 6th day after introduction of the spores. Infection of large numbers of seedlings was easily obtained by this technique, which is now being used for assessing the effects of chemicals in controlling clubroot.