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Strains of Botrytis allii resistant to chlorinated nitrobenzenes
Author(s) -
PRIEST D.,
WOOD R. K. S.
Publication year - 1961
Publication title -
annals of applied biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.677
H-Index - 80
eISSN - 1744-7348
pISSN - 0003-4746
DOI - 10.1111/j.1744-7348.1961.tb03637.x
Subject(s) - fungicide , biology , hypha , strain (injury) , agar , mycelium , nitrobenzene , microbiology and biotechnology , botrytis , botrytis cinerea , horticulture , bacteria , biochemistry , genetics , anatomy , catalysis
SUMMARY When Botrytis allii was grown in nutrient agar in the presence of vapour of penta‐chloronitrobenzene (PCNB), or isomers of tetrachloronitrobenzene (TCNB), a slow‐growing mycelium of distorted hyphae was produced on which sporulation was much reduced or suppressed. After periods which differed with the different fungicides, variants were produced which in the absence of fungicide closely resembled the parent but which, in the presence of fungicide, grew much more rapidly than the parent, and produced hyphae of normal appearance. A strain produced in response to one fungicide was more resistant than the parent to each of the other three fungicides, but the extent of this resistance depended on the origin of the strain. The most resistant was the 2,3,4,6‐TCNB‐resistant strain, followed in turn by the 2,3,5,6‐TCNB‐, 2,3,4,5‐TCNB‐, and PCNB‐resistant strains. In liquid culture, apart from the fact that fungistatic effects were less pronounced, the results were similar to those obtained on agar; the resistant strains utilized carbohydrate more efficiently than did the parent strain in the presence of each of the fungicides. The strains resistant to PCNB and TCNB were also resistant, in varying degrees, to diiodo‐, dibromo‐ and dichloronitrobenzenes. The diiodo‐ and dibromo‐ compounds were less active against B. allii than was TCNB, whereas the dichloro‐ compounds were more active and in certain conditions killed inocula. Resistant strains were also resistant to benzene, to 2,3,5,6‐tetrachloronitroaniline, and to 2,6‐dichloro‐4‐nitro‐aniline, the active principle of ‘Allisan’. Resistant strains retained their resistance for at least 18 months under ordinary conditions of laboratory subculturing, and were as pathogenic to onions as was the parent strain.